Compensatory Guaiacyl Lignin Biosynthesis at the Expense of Syringyl Lignin in 4CL1-Knockout Poplar.
Identifieur interne : 000504 ( Main/Exploration ); précédent : 000503; suivant : 000505Compensatory Guaiacyl Lignin Biosynthesis at the Expense of Syringyl Lignin in 4CL1-Knockout Poplar.
Auteurs : Chung-Jui Tsai [États-Unis] ; Peng Xu [États-Unis] ; Liang-Jiao Xue [États-Unis] ; Hao Hu [États-Unis] ; Batbayar Nyamdari [États-Unis] ; Radnaa Naran [États-Unis] ; Xiaohong Zhou [États-Unis] ; Geert Goeminne [Belgique] ; Ruili Gao [États-Unis] ; Erica Gjersing [États-Unis] ; Joseph Dahlen [États-Unis] ; Sivakumar Pattathil [États-Unis] ; Michael G. Hahn [États-Unis] ; Mark F. Davis [États-Unis] ; John Ralph [États-Unis] ; Wout Boerjan [Belgique] ; Scott A. Harding [États-Unis]Source :
- Plant physiology [ 1532-2548 ] ; 2020.
Abstract
The lignin biosynthetic pathway is highly conserved in angiosperms, yet pathway manipulations give rise to a variety of taxon-specific outcomes. Knockout of lignin-associated 4-coumarate:CoA ligases (4CLs) in herbaceous species mainly reduces guaiacyl (G) lignin and enhances cell wall saccharification. Here we show that CRISPR-knockout of 4CL1 in poplar (Populus tremula × alba) preferentially reduced syringyl (S) lignin, with negligible effects on biomass recalcitrance. Concordant with reduced S-lignin was downregulation of ferulate 5-hydroxylases (F5Hs). Lignification was largely sustained by 4CL5, a low-affinity paralog of 4CL1 typically with only minor xylem expression or activity. Levels of caffeate, the preferred substrate of 4CL5, increased in line with significant upregulation of caffeoyl shikimate esterase1 Upregulation of caffeoyl-CoAO-methyltransferase1 and downregulation of F5Hs are consistent with preferential funneling of 4CL5 products toward G-lignin biosynthesis at the expense of S-lignin. Thus, transcriptional and metabolic adaptations to 4CL1-knockout appear to have enabled 4CL5 catalysis at a level sufficient to sustain lignification. Finally, genes involved in sulfur assimilation, the glutathione-ascorbate cycle, and various antioxidant systems were upregulated in the mutants, suggesting cascading responses to perturbed thioesterification in lignin biosynthesis.
DOI: 10.1104/pp.19.01550
PubMed: 32139476
PubMed Central: PMC7210618
Affiliations:
- Belgique, États-Unis
- Attique (région), Colorado, Géorgie (États-Unis), Province de Flandre-Orientale, Région flamande, Tennessee, Wisconsin
- Athènes, Gand
- Université de Gand
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Harding</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32139476</idno><idno type="pmid">32139476</idno><idno type="doi">10.1104/pp.19.01550</idno><idno 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Athens</wicri:regionArea><placeName><settlement type="city">Athènes</settlement><region nuts="2" type="region">Attique (région)</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Plant Biology, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Xu, Peng" sort="Xu, Peng" uniqKey="Xu P" first="Peng" last="Xu">Peng Xu</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Hu, Hao" sort="Hu, Hao" uniqKey="Hu H" first="Hao" last="Hu">Hao Hu</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Nyamdari, Batbayar" sort="Nyamdari, Batbayar" uniqKey="Nyamdari B" first="Batbayar" last="Nyamdari">Batbayar Nyamdari</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Naran, Radnaa" sort="Naran, Radnaa" uniqKey="Naran R" first="Radnaa" last="Naran">Radnaa Naran</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Zhou, Xiaohong" sort="Zhou, Xiaohong" uniqKey="Zhou X" first="Xiaohong" last="Zhou">Xiaohong Zhou</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea><wicri:noRegion>9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Ruili" sort="Gao, Ruili" uniqKey="Gao R" first="Ruili" last="Gao">Ruili Gao</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Biochemistry, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><affiliation wicri:level="2"><nlm:affiliation>National Renewable Energy Laboratory, Golden, Colorado 80401.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>National Renewable Energy Laboratory, Golden</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Dahlen, Joseph" sort="Dahlen, Joseph" uniqKey="Dahlen J" first="Joseph" last="Dahlen">Joseph Dahlen</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Pattathil, Sivakumar" sort="Pattathil, Sivakumar" uniqKey="Pattathil S" first="Sivakumar" last="Pattathil">Sivakumar Pattathil</name><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Complex Carbohydrate Research Center, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Plant Biology, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Complex Carbohydrate Research Center, University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><affiliation wicri:level="2"><nlm:affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>National Renewable Energy Laboratory, Golden, Colorado 80401.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>National Renewable Energy Laboratory, Golden</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Department of Biochemistry, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison</wicri:cityArea></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent</wicri:regionArea><wicri:noRegion>9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name><affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Warnell School of Forestry and Natural Resources, University of Georgia, Athens</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, University of Georgia, Athens</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lignin biosynthetic pathway is highly conserved in angiosperms, yet pathway manipulations give rise to a variety of taxon-specific outcomes. Knockout of lignin-associated <i>4</i>-<i>coumarate</i>:<i>CoA ligases</i> (<i>4CLs</i>) in herbaceous species mainly reduces guaiacyl (G) lignin and enhances cell wall saccharification. Here we show that CRISPR-knockout of <i>4CL1</i> in poplar (<i>Populus tremula</i> × <i>alba</i>) preferentially reduced syringyl (S) lignin, with negligible effects on biomass recalcitrance. Concordant with reduced S-lignin was downregulation of <i>ferulate 5</i>-<i>hydroxylases</i> (<i>F5Hs</i>). Lignification was largely sustained by 4CL5, a low-affinity paralog of 4CL1 typically with only minor xylem expression or activity. Levels of caffeate, the preferred substrate of 4CL5, increased in line with significant upregulation of <i>caffeoyl shikimate esterase1</i> Upregulation of <i>caffeoyl</i>-<i>CoA</i><i>O</i>-<i>methyltransferase1</i> and downregulation of <i>F5Hs</i> are consistent with preferential funneling of 4CL5 products toward G-lignin biosynthesis at the expense of S-lignin. Thus, transcriptional and metabolic adaptations to <i>4CL1</i>-knockout appear to have enabled 4CL5 catalysis at a level sufficient to sustain lignification. Finally, genes involved in sulfur assimilation, the glutathione-ascorbate cycle, and various antioxidant systems were upregulated in the mutants, suggesting cascading responses to perturbed thioesterification in lignin biosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32139476</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>183</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>05</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Compensatory Guaiacyl Lignin Biosynthesis at the Expense of Syringyl Lignin in <i>4CL1</i>-Knockout Poplar.</ArticleTitle><Pagination><MedlinePgn>123-136</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.19.01550</ELocationID><Abstract><AbstractText>The lignin biosynthetic pathway is highly conserved in angiosperms, yet pathway manipulations give rise to a variety of taxon-specific outcomes. Knockout of lignin-associated <i>4</i>-<i>coumarate</i>:<i>CoA ligases</i> (<i>4CLs</i>) in herbaceous species mainly reduces guaiacyl (G) lignin and enhances cell wall saccharification. Here we show that CRISPR-knockout of <i>4CL1</i> in poplar (<i>Populus tremula</i> × <i>alba</i>) preferentially reduced syringyl (S) lignin, with negligible effects on biomass recalcitrance. Concordant with reduced S-lignin was downregulation of <i>ferulate 5</i>-<i>hydroxylases</i> (<i>F5Hs</i>). Lignification was largely sustained by 4CL5, a low-affinity paralog of 4CL1 typically with only minor xylem expression or activity. Levels of caffeate, the preferred substrate of 4CL5, increased in line with significant upregulation of <i>caffeoyl shikimate esterase1</i> Upregulation of <i>caffeoyl</i>-<i>CoA</i><i>O</i>-<i>methyltransferase1</i> and downregulation of <i>F5Hs</i> are consistent with preferential funneling of 4CL5 products toward G-lignin biosynthesis at the expense of S-lignin. Thus, transcriptional and metabolic adaptations to <i>4CL1</i>-knockout appear to have enabled 4CL5 catalysis at a level sufficient to sustain lignification. Finally, genes involved in sulfur assimilation, the glutathione-ascorbate cycle, and various antioxidant systems were upregulated in the mutants, suggesting cascading responses to perturbed thioesterification in lignin biosynthesis.</AbstractText><CopyrightInformation>© 2020 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>Chung-Jui</ForeName><Initials>CJ</Initials><Identifier Source="ORCID">0000-0002-9282-7704</Identifier><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602 cjtsai@uga.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Peng</ForeName><Initials>P</Initials><Identifier Source="ORCID">0000-0001-8007-9879</Identifier><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Liang-Jiao</ForeName><Initials>LJ</Initials><Identifier Source="ORCID">0000-0003-1766-5298</Identifier><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Hao</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0003-4986-2034</Identifier><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nyamdari</LastName><ForeName>Batbayar</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naran</LastName><ForeName>Radnaa</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Xiaohong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Goeminne</LastName><ForeName>Geert</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0002-0337-2999</Identifier><AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Ruili</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gjersing</LastName><ForeName>Erica</ForeName><Initials>E</Initials><Identifier Source="ORCID">0000-0002-2073-7224</Identifier><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, Colorado 80401.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dahlen</LastName><ForeName>Joseph</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pattathil</LastName><ForeName>Sivakumar</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0003-3870-4137</Identifier><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hahn</LastName><ForeName>Michael G</ForeName><Initials>MG</Initials><Identifier Source="ORCID">0000-0003-2136-5191</Identifier><AffiliationInfo><Affiliation>Department of Plant Biology, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark F</ForeName><Initials>MF</Initials><Identifier Source="ORCID">0000-0003-4541-9852</Identifier><AffiliationInfo><Affiliation>Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, Colorado 80401.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-6093-4521</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin 53726.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials><Identifier Source="ORCID">0000-0003-1495-510X</Identifier><AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Vlaams Instituut voor Biotechnologie, UGent Center for Plant Systems Biology, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harding</LastName><ForeName>Scott A</ForeName><Initials>SA</Initials><Identifier Source="ORCID">0000-0001-5098-2370</Identifier><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Genetics, University of Georgia, Athens, Georgia 30602.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>02</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32139476</ArticleId><ArticleId IdType="pii">pp.19.01550</ArticleId><ArticleId IdType="doi">10.1104/pp.19.01550</ArticleId><ArticleId IdType="pmc">PMC7210618</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell. 2012 Sep;24(9):3506-29</Citation><ArticleIdList><ArticleId IdType="pubmed">23012438</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2017 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(région)</li><li>Colorado</li><li>Géorgie (États-Unis)</li><li>Province de Flandre-Orientale</li><li>Région flamande</li><li>Tennessee</li><li>Wisconsin</li></region><settlement><li>Athènes</li><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><country name="États-Unis"><region name="Attique (région)"><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name></region><name sortKey="Dahlen, Joseph" sort="Dahlen, Joseph" uniqKey="Dahlen J" first="Joseph" last="Dahlen">Joseph Dahlen</name><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Gao, Ruili" sort="Gao, Ruili" uniqKey="Gao R" first="Ruili" last="Gao">Ruili Gao</name><name sortKey="Gao, Ruili" sort="Gao, Ruili" uniqKey="Gao R" first="Ruili" last="Gao">Ruili Gao</name><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><name sortKey="Hahn, Michael G" sort="Hahn, Michael G" uniqKey="Hahn M" first="Michael G" last="Hahn">Michael G. Hahn</name><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name><name sortKey="Hu, Hao" sort="Hu, Hao" uniqKey="Hu H" first="Hao" last="Hu">Hao Hu</name><name sortKey="Hu, Hao" sort="Hu, Hao" uniqKey="Hu H" first="Hao" last="Hu">Hao Hu</name><name sortKey="Naran, Radnaa" sort="Naran, Radnaa" uniqKey="Naran R" first="Radnaa" last="Naran">Radnaa Naran</name><name sortKey="Nyamdari, Batbayar" sort="Nyamdari, Batbayar" uniqKey="Nyamdari B" first="Batbayar" last="Nyamdari">Batbayar Nyamdari</name><name sortKey="Pattathil, Sivakumar" sort="Pattathil, Sivakumar" uniqKey="Pattathil S" first="Sivakumar" last="Pattathil">Sivakumar Pattathil</name><name sortKey="Pattathil, Sivakumar" sort="Pattathil, Sivakumar" uniqKey="Pattathil S" first="Sivakumar" last="Pattathil">Sivakumar Pattathil</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name><name sortKey="Xu, Peng" sort="Xu, Peng" uniqKey="Xu P" first="Peng" last="Xu">Peng Xu</name><name sortKey="Xu, Peng" sort="Xu, Peng" uniqKey="Xu P" first="Peng" last="Xu">Peng Xu</name><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name><name sortKey="Xue, Liang Jiao" sort="Xue, Liang Jiao" uniqKey="Xue L" first="Liang-Jiao" last="Xue">Liang-Jiao Xue</name><name sortKey="Zhou, Xiaohong" sort="Zhou, Xiaohong" uniqKey="Zhou X" first="Xiaohong" last="Zhou">Xiaohong Zhou</name></country><country name="Belgique"><region name="Région flamande"><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name></region><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Goeminne, Geert" sort="Goeminne, Geert" uniqKey="Goeminne G" first="Geert" last="Goeminne">Geert Goeminne</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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